Genetic diversity of pinto and fresh bean (Phaseolus vulgaris L.) germplasm collected from Erzincan province of Turkey by inter-primer binding site (iPBS) retrotransposon markers

Abstract

In this study, genetic diversity among 71 bean genotypes (41 pinto beans and 30 fresh bean genotypes) and 4 commercialcultivars (Aleyna, Gina, Perolar, and Serra) commonly grown at Erzincan province in Turkey were investigated. The iPBS (inter-primerbinding site) markers were used to investigate the genetic diversity of the genotypes. Polymorphism ratio was identified as 100% in allprimers. Twenty-seven primers were studied and number of alleles in primers varied between 13–69 (with an average value of 37.14).According to cluster analysis based on iPBS data, genotypes were divided into 2 groups. Polymorphic information content (PIC) valuesvaried between 0.19 (iPBS 2077) and 0.42 (iPBS 2381, iPBS 2385, and iPBS 2389) with an average PIC value of 0.33. According to geneticstructure analysis, genotypes were divided into 2 subpopulations. Majority of the genotypes of the regions from where beans werecollected were placed in the same groups.