Expression of roundabout receptor family members 1 and 2 in laryngeal squamous cell carcinoma and correlation with clinical and pathological parameters

Abstract

Objectives: This study aims to investigate whether there is a role of ROBO-1, ROBO-2 and TGM-3 gene expression levels in thedevelopment of laryngeal cancer.Patients and Methods: The study was completed with 29 patients who underwent total or partial laryngectomy due to squamouscelllaryngeal cancer. Gene expression analysis was performed by quantitative real-time polymerase chain reaction (qRT-PCR).Expression ratios were transformed into fold changes and reported as relative expression. Obtained fold changes were comparedbetween different tumor grades in addition to normal tissue samples.Results: The ROBO-1, ROBO-2 and TGM-3 genes were expressed at a lower level than the control group.Conclusion: Our study results showed that there was no correlation between ROBO-1, ROBO-2 and TGM-3 gene expression anddevelopment of laryngeal cancer.