Determination of triacylglycerols and fatty acid composition of Chilopsis linearis seed oil by reverse-phase high-performance liquid chromatography

Abstract

In the present work, the compositions of the 27 triacylglycerols (TAGs) as well as fatty acid composition ofChilopsis linearis seed oil were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) withdiode array and mass spectrometric detections. It was shown that the main conjugated acid in the seed oil is a catalpicacid, (9E,11E,13Z)-octadeca-9,11,13-trienoic acid, while two unusual fatty acids have the parameters satisfying theliterature data for (10E,12E)-octadeca-10,12-dienoic and (9E,12E)-octadeca-9,12-dienoic acids. For the two conjugatedacid substituents the isosbestic point (at 234 nm) was found and was used to calculate the ratios of TAGs with differentchromophores in the oil, taking into account the peak areas on the oil chromatogram.