IN-VITRO INVESTIGATION OF DONKEY MILK EFFICACY AGAINST STANDARD STAPHYLOCOCCUS AUREUS STRAINS

Abstract

Donkey milk, which is very similar to breast milk in terms of protein profile and lactosecontent, has been used for therapeutic purposes since ancient times. Different studies report itsantimicrobial activity against an important human pathogen such as S. aureus. Therefore, in our study,we aimed to examine the antimicrobial efficacy of donkey milk against standard MRSA and MSSAstrains in-vitro environment. In our study, in-vitro antimicrobial efficacy of donkey milk obtained from53 different donkeys against S. aureus ATCC 43300 (MRSA) and S. aureus ATCC 29213 (MSSA) wasinvestigated by broth microdilution method. Donkey milk mixed with TSB medium at different serialdilution rates was studied in 96 microwell plates. When the effectiveness of different concentrations ofdonkey milk added to TSB medium in vitro against ATCC MRSA and MSSA strains was examined, nosuppression was observed, and growth was found to be higher than the control results after 24 hours ofincubation at high milk concentrations. In conclusion, according to the results of our study, noantimicrobial effect of donkey milk was observed in-vitro against standard MRSA and MSSA strains.This situation may be caused by the fact that S. aureus strains may have different virulence abilities, aswell as the differences in protein structure in milk between species.