Synthesis of an Antimicrobial Thio-anthraquinone Compound to Produce Biodegradable Electrospun Mats for Tissue Engineering Purposes

Abstract

In this work, a novel S-substituted bioactive anthraquinone compound is synthesized with anew, easy and less energetic reaction methodfrom 1-chloro-9,10-dihydrodiagnosisxy-anthraquinoneand butyl-3-mercaptopropionate (Compound 3) for the first time in the literature. The synthesizedstructure is purified by column chromatography and then characterized with various spectroscopicmethods (NMR, MS, FT-IR, UV). The investigation of the antimicrobial properties of the purifiedstructure reveals remarkable biological properties. Compound 3 is not only effective against yeasts andfungi but also displays significant inhibitory effects on the growth of the tested Gram positive bacteriasimiliar to that of a positive control (Gentamicin). Subsequently, biodegradable electrospun mats areproduced via electrospinning method for their usage as a biomaterial. Structural (FTIR), morphological(FEG-SEM) biological (antimicrobial and in-vitro tests) and mechanical (tensile testing)characterizations are conducted for these nanobiomaterials. Presenting an advantage of the novelantimicrobial compound, all produced electrospun nanobiocomposites exhibit remarkable cell viability%and mechanical properties as the amount of Compound 3 increases. Cell viability values are 95% orgreater for all polymeric nanocomposites whereas, the best cell viability% and mechanical proprties areobtained for PCL-8% Compound3 composite. The obtained electrospun mats are good candiates forbiomaterials for tissue engineering purposes and wound healing materials with a purposeful compoundsynthesis and a subsequent nanobiocomposite production.