Supercritical Carbondioxide Extraction of Lavandula Officinalis (Lavender) and Hypericum Perforatum (Centaury) Plants Grown in Mersin Region: Investigation of Antioxidant and Antibacterial Activities of Extracts and Usage as Cosmetic Preservatives in Creams

Abstract

The extraction of Lavandula Officinalis (lavender) and Hypericum Perforatum (centaury) plantsgrown in Mersin region were extracted by supercritical carbon dioxide extraction system (P=100 bar, T=40oC). The chemical compositions of the lavender and centaury extracts were analyzed by GasChromatography–Mass Spectrometry (GC-MS). For antioxidant activity experiments, 1,1-diphenyl-2-picrylhidrazine (DPPH) radical was used in radical effect tests. For antimicrobial activity studies, Bacillussubtilis, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa andStreptococcus pneumoniae with Nutrient Agar Broth (NA) and Eosin Methylene-blue lactose sucrose agar(EMB) broth were used. For determining antimicrobial effect of plant extracts, diffusion method was used.Antibacterial and antioxidant properties of the obtained extracts were examined and have been determinedthat the resulting extracts have significant antioxidant and antimicrobial effects. The extracts were alsoused in cosmetic cream formulas as protectives. Effective results have also been determined in antibacterialactivity studies of creams after 6 months.