Antioxidant and Anticholinesterase Properties of Sideritis perfoliata subsp. athoa (Papan. & Kokkini) Baden and Sideritis trojana Bornm. Teas from Mount Ida-Turkey and Their Phenolic Characterization by LCMS/MS

Abstract

The phenolic profile, antioxidant, and anticholinesterase activities of teas prepared from twoSideritis (L.) species: Sideritis perfoliata subsp. athoa (Papan. & Kokkini) Baden and Sideritis trojanaBornm., collected from Mount Ida (Kaz Dağı) Turkey, were determined. The teas were prepared by infusionand decoction methods. The quantitative amounts of the phenolic contents were determined by liquidchromatography-tandem mass spectrometry (LC-MS/MS). The significant phytochemicals were found to befumaric acid for infusion and decoction samples of S. trojana and infusion of S. perfoliata subsp. athoa,chlorogenic acid for decoction of S. perfoliata subsp. athoa. The tea samples prepared by the decoctionmethod were found to be rich in phenolics. Apart from the fact that the decoction sample of S. trojanaexhibited the best antioxidant effect in 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching andcupric (Cu2+) ion reducing power assay (CUPRAC) methods among the tested samples at all concentrations,and showed significant inhibition effect at 200 μg/mL against acetylcholinesterase and butyrylcholinesteraseenzymes (59.74%, 64.99%, respectively).