Evaluation of IFN-γ level in peripheral blood mononuclear cell of childhood tuberculosis treated by lactic acid bacteria multi cultures

Abstract

At least a half million children in the world are suffering from TB every year and 64.000 children diedfrom TB in 2011. Previous works indicated the ability of probiotic in stimulating the production of IL-12 and IFN-γwhich subsequently increased the role of type Th1 responses and improved the balance of Th1-Th2. Antigencombination of Mycobacterium tuberculosis (Mtb) and Lactic Acid Bacteria (LAB) initiated synergistic increase of IFN-γbigger than the level induced by Mtb or LAB. This study aimed to analyze the administration effects of LAB multicultures (Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Bifidobacterium bifidum, Bifidobacteriumanimalis, Lactobacillus plantarum, Streptococcus thermophilus) to the secretion of IFN-γ on peripheral blood mononuclearcell (PBMC) culture supernatant of childhood tuberculosis patients under medication. The PBMC of the patientsisolated from whole blood was culture-treated in 4 groups: (1) without treatment (-MtbCell, -LAB), (2) incubation withMtb, without LAB administration (+MtbCell, -LAB), (3) with LAB administration, without incubation with Mtb(-MtbCell, +LAB), and (4) incubation with Mtb and LAB administration (+MtbCell, +LAB), to subsequently be taken thesupernatant and to examine IFN-γ by using enzyme-linked immunosorbent assay. There was a significant difference inIFN-γ assay (p = 0.006) between group (1) compared to the group (4). Addition of the LAB multi cultures increasedthe secretion of IFN-γ supernatant on the PBMC culture supernatant of childhood tuberculosis patients undermedication. The LAB increased 6.31% of IFN-γ level, while treatment with LAB and Mtb increased IFN-γ level up to15.79% compared to IFN- γ level in the PBMC without treatment.