Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect

Abstract

Wound healing involves many complex mechanisms, and many growth factors are effective in thisprocess. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation,proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factorstimulates (TGF-β) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradationproduces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine thetherapeutic effect of the TGF-β1with the positive effect of the drug delivery system including PLGA nanoparticles (TGFβ- PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitoryeffect of TGF-β1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferativeeffect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-β1 has an inhibitory effect onkeratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay.