HPTLC-DPPH• and HPTLC-tyrosinase methods for hot water-soluble contents of kumquat, limequat and Mexican lime fruit powders

Abstract

In the present study, small Citrus fruits which are cultivated in our country and have an economic value were chosen as research materials. It was aimed to do fingerprint analysis of the fruits by HPTLC method. DPPH• scavenging and antityrosinase potentials were determined in vitro. Extracts showed noticable activities, but these effects were not comparable with positive controls. HPTLC-bioautographic assays and Mass Spectrometry analysis were employed to identify bioactive compound in the extracts. Phloretin-di-C-glucoside was tentatively identified as DPPH• scavenging and antityrosinase active compound in kumquat and limequat extracts.