Determination of dihydrocapsaicin adulteration in dietary supplements using LC-MS/MS

Abstract

Adulteration of dietary supplements are serious threats to public health in all around of world. Nowadays,in order to obtain the intended effect from natural remedies, the adulteration of natural products via artificial orharmful chemicals are increasing. A rapid LC-MS/MS method for determination of capsaicin and dihydrocapsaicinin dietary supplements via Fortis C18 (150 x 3 mm; 3 µm) column at 25 °C was developed and validated in amixture of mobile phase A (1 % formic acid solution and 0.1 % ammonium formate in water) and B (1 % formicacid solution and 0.1 % ammonium formate in methanol) with gradient program at a flow rate of 0.35 mL/min. Therecoveries of developed methods weytre obtained in the range of % 95.4-106.3 for capsaicin and % 96.3-108.9 fordihydrocapsaicin. This study shows that synthetic compound dihydrocapsaicin is widely use in dietary supplementsproducts for adulteration to increase the effects of product without any declaration.