Koyun Embriyolarının Dondurulmasında Farklı Soğutma Hızlarının Embriyoların Canlılığı veGebelik Oranları Üzerindeki Etkisi

Abstract

In the first stage of the study, oocytes obtained from ovaries from abbatoir (n=2990) were incubated in maturation medium for 24 h. The maturated oocytes were left for in vitro fertilization (IVF) for 20 hours. The cleavaged embryos (n=1305) were left for in vitro culture (IVC) for six days. Then morula-blastocyst stage embryos divided randomly in three groups (Grup I: 0.5°C/min, Grup II: 0.8°C/min, Grup III: 1°C/min). Embryos in each group (n=50) were frozen at different cooling rates in 1.5 M ethylene glycol containing freezing medium. As a result, 0.5°C/min cooling rate group was found as the most successful group (P