Leishmania major promastigot hücrelerine karşı makrofajlardan nitrojen radikali ve tümör nekroz faktörü salgılanması

Production of nitrogen radicals and tumor necrosis factor by macrophages againts Leishmania major promastigotes

Özet

Leishmania major promastigot hücrelerinin fare periton makrofajlarından (PMfy) nitrojen radikali (NO2) ve tümör nekroz faktör (TNF) salgılatma kapasitesi araştırıldı. PM kültür süpematantlarındaki NO2 düzeyi Griess ajanıyla oluşan reaksiyon sonrasında 550 nm'de spektrofotometrik yöntemle ölçüldü. PM kültür süpernatanlarıyla karşılaştırıldığında, 2x104 promastigot hücresi/ml dozunda bile PM0 'lardan anlamlı düzeyde (p

Abstract

The capacities of Leishmania major promastigote cells to induce nitrogen radicals (NO2) and TNF from murine peritoneal macrophages (PM) were investigated. NO2 levels in the PM culture supernatants were determined spectrophotometrically at 550 nm following reaction with the Griess reagent. Tumor necrosis factor (TNF) levels in the PM(j> culture supernatants were determined by using L929 bioassay. Elevated NO2 and TNF release was observed in PM culture supernatants after a 36 h incubation with L. major promastigote cells. It was observed that releases of NO2 and TNF from PMtp against L. major promastigote cells increased in a dose dependent manner. Even at a concentration of 2x104 promastigote cells/ml, significant (p