The inhibitory effects of plant extracts, vitamins and amino acids on myeloperoxidase activity

Abstract

Background and Aims: Myeloperoxidase (MPO, EC 1.11.2.2) is a vital antimicrobial enzyme, having a crucial role in host defense. Obstructing the activity of MPO is a possible pharmacological approach for the hindrance and management of a widearray of inflammatory illnesses. Consequently, blocking the activity of MPO is a potential pharmacological strategy for prevention and treatment of a broad range of inflammatory diseases.Methods: In our study, inhibitory effects of 6 different sulfur containing plant extracts, 16 different vitamins and amino acidswere studied for MPO inhibitory activities. The MPO enzyme activity was determined spectrophotometrically according to themethod of Wei and Frankel.Results: Among the aqueous plant extracts, black cabbage extract having IC50=0.92±0.07 mM showed the highest inhibition. Among the vitamins and amino acids studied, the highest MPO enzyme inhibition was exhibited by ascorbic acid withIC50=0.01±0.003 and cysteine with IC50=1.09±0.73 mM.Conclusion: Based on the outcomes, it was observed that all the examined plant extracts, vitamins and amino acids inhibitedMPO enzyme at certain ratios.