Core shell column in high-performance liquid chromatography for the determination of polar compounds; troxerutin and carbazochrome

Abstract

Background and Aims: The combination of troxerutin and carbazochrome has been found efficacious for non-surgical patients with acute uncomplicated hemorrhoids. Therefore it is essential to develop a simultaneous analysis method of thosedrug substances. This study explains a simple and selective HPLC method to analyze troxerutin and carbazohrome in tablets.Methods: The analysis was carried out using a core-shell pentafluorophenyl propyl polar column with a mobile phase consisting of (MeOH; % 5 HAc), (99.5:0.5 - V/V) at a flow rate of 0.25 mL/min. UV detection was set to 350 nm.Results: The standard calibration curve was established between 1.00-20.00 µg/mL for troxerutin; 0.05-1.00 µg/mL for carbazochrome. LOD and LOQ were found to be 0.65 µg/mL and 1.00 µg/mL for troxerutin and 0.01 µg/mL and 0.05 µg/mL forcarbazochrome, respectively.Conclusion: The proposed method was validated for specificity, linearity, accuracy, precision, LOQ and LOD and then successfully applied for the analysis of these substances in tablets.