Spectrophotometric determinations of most commonly used statins in pharmaceutical preparations with 2,3-dichloro-5,6-dicyanobenzoquinone

Abstract

Background and Aims: In this study, simple, accurate and precise spectrophotometric methods were developed for the determination of five 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase inhibitors in pure and pharmaceuticaldosage forms.Methods: In the developed methods, atorvastatin fluvastatin, pitavastatin, rosuvastatin and simvastatin were used in thisclass of drugs called statins. The methods were based on the charge-transfer reaction of n-electron donor drugs withπ-acceptor 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ). All variables such as temperature, time, reaction medium,amount of reagent were examined for optimal formation of complexes to be given by the drugs with DDQ and optimal conditions were determined.Results: The linearity ranges for atorvastatin, fluvastatin, pitavastatin, rosuvastatin and simvastatin were found to be 0.5-50,1-6, 2.5-50, 5-25 and 5-50 µg/mL, respectively.Conclusion: The proposed methods were successfully applied to both the pure and the pharmaceutical dosage forms.