Cytotoxicity and DNA protective effects of the Terfezia and Picoa species from the eastern region of Turkey

Abstract

Background and Aims: This study aims to investigate the cytotoxicity and DNA protective effects of semi-arid, arid, or deserttruffles such as the Terfezia and Picoa species.Methods: The DNA protective effects of the Terfezia and Picoa species was evaluated using plasmid pBR322 DNA treated withUV and H2O2. The cytotoxic effects of water and methanol extracts of the Terfezia and Picoa species on H1299 and HUVEC cellswere evaluated using the MTT assay.Results: Higher concentrations of T. olbiensis and P. lefebvrei extracts have demonstrated a DNA protective effect. The waterextract of P. juniperi only at a 40 mg/mL concentration demonstrated a DNA-protective effect, whereas T. boudieri extract didnot show any DNA-protective effect at all concentrations tested. H1299 cells showed more sensitivity to the water extract ofT. olbiensis (4.66%), P. juniperi (12.04%), P. lefebvrei (21.93%), and the methanol extract of T. boudieri (20.93%). In general,the water and methanol extracts of the Terfezia and Picoa species demonstrated the least cytotoxic effects on HUVEC cells,except for the water extract of P. juniperi (38.46%).Conclusions: In conclusion, results obtained from this study show that the Terfezia and Picoa truffle species have potentialDNA protective and cancer prevention properties.