Co-application of EDDS and ZnO nanoparticles with TiO2Ag nanoparticles on rye

Abstract

The aim of this study was to determine the effects of nTiO2Ag, co-application of nZnO and nTiO2Ag and co-application of EDDS as an organic acid with nTiO2Ag on seed germination, seedling vigor, plumule and radicle length of rye. Ten seeds were placed in petri-dishes with double layer of filter paper which used as an inert material. Then 5 mL nTiO2Ag, nZnO-nTiO2Ag and EDDS-nTiO2Ag suspensions were added to every petri dish with different concentrations (control, 50, 100 and 200 mg/L). Treatment of nZnO-nTiO2Ag, especially at concentration of 50 mg/L, promoted the germination rate, seedling vigor, plumule and radicle elongation. The highest seedling vigor index (SVI) was observed at concentration of 50 mg/L nZnO-nTiO2Ag. Seedling vigor index of rye seeds was decreased after treatment of nTiO2Ag. The plumule elongation increased with treatment of all test chemicals and radicle elongation was increased nZnO-nTiO2Ag and EDDS-nTiO2Ag exposure compared to control. This is the first report on the effect of co-application of ZnO-TiO2Ag nanoparticles and co-application of EDDS-TiO2Ag nanoparticles on rye growth.