Evaluation of Chitosan /Organoclay Composite as Bone Tissue Engineering Scaffold

Abstract

I n this study, a scaffold composed of chitosan C and organoclay OC was prepared by utilizing freeze-drying method. The composition of resulting composite scaffold was evaluated using FTIR and SEM. The in vivo cytocompatibility of the composite was evaluated in vivo, by seeding MC3T3-E1 cells on composite scaffolds. In-vitro cell viability and proliferation were investigated using the MTT assay, while cell-scaffold constructs were evaluated using scanning electron microscopy. Preliminary in vivo experiments were also performed to evaluate tissue compatibility and ectopic boneforming potential of C/OC composite scaffolds with MC3T3-E1 cells. The findings suggest that C/OC construct supported the proliferation of MC3T3-E1 cells, presented histobiocompatibility and promoted formation of calcified matrix. Based on the results, we conclude that the characterized C/OC composite system may have potential for use in bone tissue engineering applications