Türkgeldi koyun populasyonunun bazı kalıtsal polimorfik kan proteinleri bakımından genetik yapısı

The genetic structure of some herediter polimorphic blood proteins in Türkgeldi type sheep herds

Özet

Bu çalışmada 93 baş Tiirkgeldi koyunundan alman kan örneklerinde bazı genetik olarak kontrol edilen karakteristikler incelenmiştir. Koyunların boyun toplar damarından (W. Jugularis) alınan kan örneklerinden serum ve hemolizat kısımları ayrılarak yatay nişasta jel elektroforez metodu ile senim transferrin ve hemolizat hemoglobin tipleri tayin edilmiştir. Bu çalışmada 6 transferrin alleli (Tf A, TfB, TfM, TfD, TfE, TfS) ile oluşturulan 4 homozigot (TfAA; TfBB; TfMM; TfDD) 11 heterozigot (TfAB; Tf AD; Tf AM; TfAE; TfB M; TfBE; TfBS; TfMD; TfME; TfMS; TfBD) olmak üzere toplam 15 fenotip gözlenmiştir. Hemoglobin lokusunda TfAA, TfBB, TfAB gibi üç fenotip gözlenmiştir. Hemoglobin lokusundaki A ve B allel frekansları sırasıyla 0.291 ve 0.705 olmuştur. Transferrin lokusundaki A, B, M, D, S ve E allel frekansları sırasıyla, 0.084; 0.270; 0.411; 0.197; 0.022; 0.016 olmuştur.Heterozigot Tf fenotipleri frekansı %76.4, homozigot Tf fenotipleri frekansı ise %23.6 olmuştur. En yüksek Tf fenotip frekansı % 29.2 ile BM olurken en düşük Tf fenotip frekansları ise % 1.1 ile AA, AE, BE, BS ve BD olarak tesbit edilmiştir. Araştırma sürüsü bu alleller bakımından genetik denge içerisinde bulunmuştur.

Abstract

This research has been conducted in order to determine Transferrin (Tf) and Haemoglobin (Hb) types of 93 head of Tiirkgeldi type sheep herds raised in Tiirkgeldi state farm. Five cc of blood samples taken from jugularis wein, seperated to sera and red cell lysate fraction. The seraand haemolysalis obtained were analysed with horizental starchgel electrophoresis. Six Tf alels (Tf A, TfB, TfM, TfD, TfE, TfS) have been izolated. Fifteen Tf phenotypes consisting of four homozygot (Tf-AA, Tf-BB, Tf-mm, Tf-DD) eleven hetorozygot (Tf-AB; Tf-AD; Tf-DM; Tf-AE; TF-BM; Tf-BE; Tf-BS; TF-MD; TF-ME; TF-MS; Tf-BD) types have been detected. Three haemoglobin types were found in the herd. The most frequent Hb phenotype was Hb-BB (48.4 % Hb-AB was found to be in a rate of 44.1 % in the herd the gene frequency of Hb-A allel was found as 0.295 and of the Hb-B allel tabe 0.705. 15 types of transferrin were found in the herd 4 of them were homozygot transferrin phenotypes (TfA A; TfB B; TfMM; TfDD) and 11 of them were heterozygote types (Tf-AB; Tf-AD; Tf-AM; Tf-AE; Tf-BM; Tf-BE; Tf-BS; Tf-MD; Tf- ME; Tf-MS; Tf-BD. The most common and the least common Tf fenotypes among homozygots were the Tf-BM (29.2 %) and Tf-AA; Tf-AE; Tf-BE; Tf-BE; Tf-BS; Tf-ME(% Irrespectively. The homozygot and heterozygot Tf fenotypes frequency were the % 23.6 and % 76.4, respectively. It was observed by the Hardy-Weinberg equations that there was no major deviation in the expected frequencies.