Characterization of beta-glactosidase from Alternaria alternata

Abstract

In this study, optimum conditions affecting the activity of beta-galactosidase enzyme from Alternaria alternata were determined and its stability was investigated. It was concluded that 0.75% ONPG concentration is suitable for high beta-galactosidase activity. The optimum temperature and pH were found to be 50$^circ$ C and 5, respectively. The enzyme was stable between 20-50$^circ$ C in the pH range 3.0-7.0 and kept more than 50% of its original activity after 40 min at 60$^circ$ C.