Atopik Dermatitli Çocuklarda Serum Il-8, Tnf-α ve Il-6 Seviyeleri

Murat ARAL, Ekrem KİREÇÇİ, Ergül Belge KURUTAŞ, Perihan ÖZTÜRK, Mustafa ÇELİK

Özet

Girifl: Atopik dermatit (AD) T yardımcı 1 (Th1) ve T yardımcı 2 (Th2) hücreleri arasında bir dengesizlikle ilişkilidir. Özellikle çocukları etkileyen, kronik, yineleyici inflamatuvar bir deri hastalığıdır. Son zamanlarda immünopatogenezi ile ilgili yoğun araştırmalar yapılmış ve yeni görüşler öne sürülmüştür. Sitokinlerin atopik dermatitin immünopatogenezindeki rolüne ilişkin çalışmalar genellikle in vitro gözlemlere dayanmaktadır ve daha tam olarak aydınlatılamamıştır. Bu çalışmada, ADli çocuk hastalarda IL-8, TNF-α ve IL-6nın serum seviyeleri ve bu parametreler ile hastalığın şiddeti arasındaki ilişki araştırıldı. Gereç ve Yöntem: AD şiddeti, atopik dermatit ağırlık ölçeği (SCORAD) ile değerlendirildi. IL-8, TNF-α ve IL-6nın serum seviyeleri ELISA yöntemi ile çalışıldı. Bulgular: Sağlıklı kontrollere kıyasla, ADli hastalarda serum IL-8 (p

Abstract

Introduction: Atopic dermatitis (AD) is associated with an imbalance between T helper 1 (Th1) and T helper 2 (Th2) cells. It is chronic relapsing inflammatory skin disease affecting especially the children. Recently, it has been intensively studied and new aspects regarding the immunopathogenesis are suggested. Studies about the role of cytokines on formation of atopic diseases are rather new and most of them are based on in vitro observations. It is not completely clear yet how cytokines regulate diseases in vivo and studies about this subject are rather limited. In this study; the serum levels of IL-8, TNF-α, IL-6 and the relationship between these parameters and the disease severity in a group of children with AD were investigated. Materials and Methods: The severity of AD was assessed by the same dermatologist using the Scoring Atopic Dermatitis (SCORAD) index system. IL-8, TNF-α, and IL-6 levels were measured by ELISA method. Results: Serum levels of IL-8, TNF-α and IL-6 were determined and were found statistically significantly higher in patients than controls. A statistically significant correlation between serum levels of IL-8, TNF-α, and IL-6 and SCORADs in children with AD was determined. Conclusion: These results show that serum levels of IL-8, TNF-α, and IL-6 may be used as important markers in the assessment of disease severity and follow-up of child patients with AD. As a result, the role of cytokines and the relationship between cytokines in the immunopathogenesis of AD are rather complex and still not clearly clarified, further investigations are required to understand this complex process. (Jour nal of Cur rent Pediatrics 2012; 10: 50-4)α,