Dergiler / GORM:Gynecology Obstetrics & Reproductive Medicine / 1997 / Cilt: 3 - Sayı: 3
Tamoxifen ile progesterone reseptör indüksiyonu: Endometrial kanser tedavisine adapte edilebilir faz I deneysel çalışma
- Sayfa
- 448–452
- DOI
- —
Özet
OBJECTIVE: To investigate the usability of tamoxifen in increasing the effectiveness of progesterone in the treatment of endometrial adenocarcinoma and to establish an. experimental animal model related with this subject. MATERIALS and METHODS: A total of 22 Sprague-Oawley rats weighing between 250-30Ü gr. are surgically castrated to keep their endocrine functions at the same level. Then all the rats were given estrogen and progesterone, in doses adjusted to their body weights, to create equal cycles; and then they are divided into two groups as tamoxifen and control. Estrogen and progesterone receptor levels of the tamoxifen group are compared with the control group which did not receive any treatment. Receptor levels are detected by immunohistochemical method. The stained nuclei are counted and their ratio to the total nucleus number is measured.. RESULTS: The mean percentage (mean ± SD) of endometrial estrogen and progesterone receptors in the rats which received tamoxifen were 81.9 ± 6.0 % and 77.7 ± 8.5 %, respectively. These values were significantly higher than the control group (66.1 ± 6.0 and 69.9 ± 6.0, respectively) (p
Abstract
OBJECTIVE: To investigate the usability of tamoxifen in increasing the effectiveness of progesterone in the treatment of endometrial adenocarcinoma and to establish an. experimental animal model related with this subject. MATERIALS and METHODS: A total of 22 Sprague-Oawley rats weighing between 250-30Ü gr. are surgically castrated to keep their endocrine functions at the same level. Then all the rats were given estrogen and progesterone, in doses adjusted to their body weights, to create equal cycles; and then they are divided into two groups as tamoxifen and control. Estrogen and progesterone receptor levels of the tamoxifen group are compared with the control group which did not receive any treatment. Receptor levels are detected by immunohistochemical method. The stained nuclei are counted and their ratio to the total nucleus number is measured.. RESULTS: The mean percentage (mean ± SD) of endometrial estrogen and progesterone receptors in the rats which received tamoxifen were 81.9 ± 6.0 % and 77.7 ± 8.5 %, respectively. These values were significantly higher than the control group (66.1 ± 6.0 and 69.9 ± 6.0, respectively) (p