The effect of intestinal ischemia on plasma thiol/disulphide homeostasis in anexperimental study

Abstract

Aim: To investigate the effects of acute intestinal ischemia on plasma thiol/disulphide homeostasis (TDSH), which has been investigated in a limited number of studies in the related literature. Methods: Twenty-four rats were randomized into control (operation without ischemia, GIS), and ischemiagroups (GII-60, GIII-180). For ischemia, the superior mesenteric artery was sutured and the rats were exposedto 60 and 180 minutes of intestinal ischemia, respectively. Plasma TDSH was measured in blood samplescollected at the end of the ischemia, and the pathology of ileum segments resected was evaluated.Results: The experimental ischemic conditions provided were confirmed by the total histopathological scoringsystem statistically. The levels of serum human albumin and ischemia modified albumin (IMA) in groups weredetected in quite a close range of each other. There was no found a statistically significant difference for IMAbetween groups (p>0.05). The alternations on the levels of plasma TDSH parameters were observed in thestudy. According to ischemic conditions, the thiol/disulfide ratio fluctuations were detected in the plasmaTDSH. The native thiol and total thiol levels seem to have decreased according to ischemia; no statisticaldifference was detected. In addition, the disulfide levels increasing according to ischemia either was not foundsignificant statistically (p>0.05). Conclusion: Although this study showed the oxidative balance in intestinal ischemia had affected plasmaTDSH, also it revealed that intestinal ischemia didn't create a statistically significant difference betweenplasma TDSH components.