The effect of morphine delta receptor activity on ischemic postconditioning in lung ischemia reperfusion injury

Abstract

OBJECTIVE: In the context of the physiopathology of lung damage due to ischemia andreperfusion injury, we aimed to reveal the effects of the addition of morphine sulfate to ischemicpostconditioning (PC) protocol.METHODS: In the present study, 48 Wistar albino female rats were employed. Group 1 wasaccepted as the Sham group that underwent thoracotomy through the fifth left intercostal space.Ischemia-reperfusion (IR) group: Thoracotomy and IR period. IRPC group: thoracotomy, IRperiod and ischemic PC. In IRPC3 and IRPC30 groups, in addition to ischemic PC different dosesof morphine sulfate (3 μmol and 30 μmol) was administered. Tumor necrosis factor (TNF)-α,interleukin (IL)-1, and IL-10 levels were measured in the biochemical assessment of the lungtissue samples obtained.RESULTS: TNF-α and IL‑1 (pro‑inflammatory cytokines) have lower values, and IL‑10 (anti‑inflammatorycytokine) have higher values both in the groups which have been subject to PC and morphine. TNF-αand IL‑1 levels in lung tissue were statistically significant between the IRPC3 group and the IR andIRPC groups. In addition, IL‑10 level in lung tissue was statistically significant between the IRPC3group and the IRPC group.CONCLUSION: In the present study conducted with experimental animals where morphine wasalso injected beside ischemic PC protocols, statistically significant differences were determinedin the lung tissue analyses when we compared pro‑inflammatory and anti‑inflammatory cytokinevalues. We firmly believe that adding morphine to the lung transplantation protocols and PC willdecrease IR damage.