Association Between Macrophages with Angiogenic Cellsand Microvascular Dysfunction in Astrocytic Glioma

Abstract

Objectives: Astrocytic gliomas are the most common primary brain tumours that developed from glial origin. Macro phages are the predominant inflammatory cells in infiltrating gliomas. The study aimed to investigate the association between circulating macrophages with tissue resident angiogenic cellsand microvascular dysfunction in astrocytic glioma. Methods: A total of 22 astrocytic glioma patients were consented from Hospital Universiti Sains Malaysia. Tumour(n=22) were sliced and stained with CD133+ and VEGFA+ angiogenic markers and counter stained with DAPI. TheSpearman rho’s test was used for the data analysis. The plasma CD68, macrophage level and von Willebrand factor levelor factor VIII level (vWF/ FVIII) was measured using Elisa Kit (CUSABIO BIOTECH CO., LTD). Results: The mean plasma CD68 macrophages was 12.48±19.98 pg/ml. The mean percentage of brain tumour tissueangiogenic cells was (1.26±0.95%), adjacent normal brain tissue angiogenic cells was (0.72±0.68%). Spearman’s rho cor relation test showed a significant correlation between brain tumour angiogenic cells and plasma CD68 macrophages(n=22) (Spearman’s rho, r=0.43, p=0.047). However no correlation was observed between plasma CD68 macrophageswith adjacent normal brain (Spearman’s rho r =0. 019, p=0. 932). In this study vWF/FVIII level was analysed and about14 patients with (mild factor level of >5%), 8 patients with (moderate factor level of 1-5%) and no patients had (severefactor level of