Effect of Citrullus colocynthis Extract on Glycated Hemoglobin Formation (In Vitro)

Abstract

Objective: Diabetes mellitus (DM) is typically a disorder of carbohydrate, fat, and protein metabolism. Itdevelops due to a lack of or loss associated with insulin and/or resistance to insulin. Regarding complicationsof chemical substance use, drugs with few complications and high-reliability tannins are needed. This studyaimed to determine the effect and mechanism of action of Citrullus colocynthis extract on the formation ofglycated hemoglobin (HbA1c).Materials and Methods: A solution containing hemoglobin and glucose was incubated for 1, 2, 3, 4, 30,and 60 days by adding Citrullus colocynthis extract or glutathione. Quantitative measurement of HbA1c wasperformed using ion-exchange chromatography. Data were analyzed using ANOVA and two-way repeatedmeasures test. A p