SIMULTANEOUS QUANTITATIVE ANALYSIS OF TWO ANTHELMINTIC DRUGS IN A VETERINARY DOSAGE FORM

Abstract

In this research article, a new chromatographic method ultra-performance liquid chromatography (UPLC) method was developed for the quantification of ivermectin (IMT)-preziquantel (PRZQ) in a two-component mixture and veterinary dosage form mixture. The separation of the studied veterinary drugs (IMT and PRZQ) was carried out on Waters Acquity ® BEH C18 column (50mm x 2.1 mm i.d., 1.7 m) using a mobile phase consisting of water-acetonitrile-methanol (10:70:20, v/v). In the chromatographic analysis, flow rate was 0.38 mL/min, and column temperature was maintained at 42 0C. The chromatographic detection of IMT and PRZQ was made at the wavelength of 220.0 and 245.0 nm, respectively. The developed UPLC method was validated and applied to real samples consisting of IMT and PRZQ. Recovery assay results were found to be 100.4 % for PRZQ and 101.8 % for IMT. The presented UPLC method is introduced as an alternative choice for the quality control of pharmaceutical preparations containing these drugs. For the quality control of pharmaceutical preparations containing these drugs, the presented UPLC method is introduced as an alternative experimental analysis choice