Antioxidant and prooxidant activity of new 1,2-diols and thiadiazoles derivatives in Saccharomyces cerevisiae yeast cells

Abstract

The present research was undertaken to determine the effect of substituted 2,2'-[(2R,3R)-2,3-dihydroxy-1,4-dioxobutane-1,4-diyl]bis(N-R-hydrazine-1-carbothioamide and (1R,2R)-1,2-bis[5-(R-amino)-1,3,4-thiadiazole-2-yl]ethane-1,2-diols on the antioxidant status of the yeast Saccharomyces cerevisiae cells. This cell serves a good eukaryotic model system for the study of molecular mechanisms of oxidative stress. The Saccharomyces cerevisiae yeast cells were treated a series of 1,2-diols and thiadiazoles compounds and the malondialdehyde (MDA) and antioxidant vitamins (A, E, C) levels in the medium were measured by HPLC-UV. In the comparison done among groups, the MDA which is an indicator of lipid peroxidation and Vitamin E concentrations were showed statistically changed in the samples. Exposure of yeaset cells to L9 showed an increase in MDA and decrease in vitamin E levels but L2 and L8 showed decrease in MDA and increase in vitamin E levels. The results showed that compounds L9 caused a considerable oxidative stress and L2 and L8 have antioxidant activity.