Determination of Antibiotic Susceptibility Profile and Intl1, blaSHV and blaTEM Genes of Raw Milk Origin Enterobacteriaceae Isolates

Abstract

In this study, a total of 68 raw milk samples were used to investigate the prevalence of Enterobacteriaceaein milk samples obtained from different dairies and supermarkets in the province of Amasya (Turkey),and to determine the antibiotic resistance profile and presence of the Intl1, blaTEM and blaSHV genes. Inthis study, isolates were obtained using a classical culture technique. The detection of antibiotic resistanceprofile was carried out using disc diffusion methods. Twelve different antibiotics, including meropenem,cefotaxime, nalidixic acid, ceftriaxone, chloramphenicol, ceftazidime, streptomycin, ampicillin,gentamicin, tetracycline, levofloxacin and trimethoprim-sulfamethoxazole were employed. Single strainpolymerase chain reaction (PCR) was created for the detection of extended spectrum beta-lactamase(ESBLs). For these aims, blaTEM and blaSHV genes were demonstrated by PCR assay. Intl1 wasdetermined by using PCR assay. As a result, 50 isolates that belong to the Enterobacteriaceae family wereobtained. A total of 41 (82%), 38 (76%), 7 (14%), 6 (12%), 2 (4%) and 1 (2%) isolates were determinedto be resistant to ampicillin, trimethoprim-sulfamethoxol, ceftazidime, cefotaxime, meropenem andnalidixic acid as well as ceftriaxone and streptomycin respectively. In addition, 49 (98%), 50 (100%) 49(98%) and 50 (100%) isolates were determined to be sensitive to chloramphenicol, gentamicintetracycline and levofloxacin respectively. Among the Enterobacteriaceae isolates, 22 (44%), 6 (12%) and2 (4%) rates of strains carried the Intl1, blaSHV and blaTEM genes, respectively. In conclusion, theresistance of Enterobacteriaceae isolates that were isolated from milk samples to many antibiotics poses apotential danger in terms of public health.