Paraoxonase 1 activity and survival in sepsis patients

Abstract

Background: Sepsis is a state of augmented oxidativestress and diminished antioxidant capacity. High den- sity lipoprotein (HDL) particles were shown to possessantioxidant and anti-inflammatory properties, as wellas Paraoxonase 1 (PON1), which is an enzyme that isalso protective against HDL oxidation. Previous stud- ies suggested a possible role of decreased PON1 activ- ity or HDL levels in sepsis patients. Aims: The present study was designed to test a hypoth- esis that higher PON1 activity and HDL-cholesterollevels could predict a better survival in sepsis patients. Study Design: Observational study. Methods: Venous blood samples were collected fromsepsis patients for HDL-cholesterol levels, PON1 ac- tivity and cytokine assays (TNF-α and IL-6) and AcutePhysiologic and Chronic Health Evaluation II (APACHEII) scores were calculated in order to weight patientsdisease severity on the day of sepsis diagnosis. Patientswere followed-up until the 28th day for any cause intra- hospital mortality. Data were statistically analyzed foreffects of study parameters on patients survival. Results: In total, 85 patients with sepsis were includedin the study. The mean age was 65.2±17.9 years and48 were male; at the end of the 28-day follow-up pe- riod, 46 survived. TNF-α (86.9±10.5 vs 118.6±16.4)and IL-6 levels (906.7±82.7 vs 1323.1±54.3) were sig-nificantly higher in non-survivors, while PON1 activity(140.7±42.3 vs 66.7±46.6) and HDL-cholesterol lev-els (43.6±8.1 vs 34.5±8.9) were significantly higher insurvivors (p