Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Abstract

Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin. Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells. Study Design: Cell culture study. Methods: DRG cells prepared from 1-day old SpragueDawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 ?M) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 ?M), dizocilpine (MK-801) (1-3 ?M) or thapsigargin (100- 300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay. Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 ?M). Nimodipine (1 ?M) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 ?M of cisplatin. Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.