Dergiler / Balkan Medical Journal / 2018 / Cilt: 35 - Sayı: 1
Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis
- Sayfa
- 30–35
- DOI
- —
Abstract
Background: The humoral system is activated andvarious cytokines are released due to infections intissues and traumatic damage. Nuclear factor-kappa Bdimers are encoded by nuclear factor-kappa B genesand regulate transcription of several crucial proteins ofinflammation such as tumour necrosis factor-alpha.Aims: To investigate the possible effect ofpolymorphisms on tumour necrosis factor-alpha serumlevels with clinical and prognostic parameters of sepsisby determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A)gene polymorphisms and tumour necrosis factor-alphaserum levels.Study Design: Case-control study.Methods: Seventy-two patients with sepsis and 104healthy controls were included in the study. In order todetermine the polymorphisms of nuclear factor-kappaB-1-94 ins/del ATTG and tumour necrosis factor-alpha(-308 G/A), polymerase chain reaction–restrictionfragment length polymorphism analysis was performedand serum tumour necrosis factor-alpha levels weredetermined using an enzyme-linked immunosorbentassay.Results: We observed no significant differences intumour necrosis factor-alpha serum levels between thestudy groups. In the patient group, an increase in thetumour necrosis factor-alpha serum levels in patientscarrying the tumour necrosis factor-alpha (-308 G/A) Aallele compared to those without the A allele was foundto be statistically significant. Additionally, an increase inthe tumour necrosis factor-alpha serum levels in patientscarrying tumour necrosis factor-alpha (-308 G/A) AAgenotype compared with patients carrying the AG or GGgenotypes was statistically significant. No significantdifferences were found in these 2 polymorphismsbetween the patient and control groups (p>0.05).Conclusion: Our results showed the AA genotype andthe A allele of the tumour necrosis factor-alpha (-308G/A) polymorphism may be used as a predictor ofelevated tumour necrosis factor-alpha levels in patientswith sepsis.