CELLULOSE MONOACETATE/NAFION (CMA/N) HYBRID NANOFIBERS AS INTERFACE FOR ELECTROCHEMICAL DNA BIOSENSORS

Abstract

Nafion/cellulose monoacetate (CMA/N) hybrid nanofibers were produced via a one-step electrospinning method. Nanofibers morphologies transformed from uniform to bead on a string defect morphology with increasing Nafion ratio in CMA/N hybrid nanofibers. The melting point of CMA was detectable at DSC measurement, but the addition of Nafion didn’t allow a proper crystallization of CMA and melting peak disappeared after the Nafion addition. Decomposition temperature decreased dramatically with the addition of Nafion into CMA/N nanofibers and decomposition took place at a broad temperature range. Differential pulse voltammetry (DPV) analyses were conducted to observe the guanine oxidation signal at neat and NH-modified DNA on the as-prepared nanofiber sensory system. Maximum oxidation signals were obtained from pure CMA nanofibers at neat ssDNA. Comparing neat DNA, signal intensity increased with the addition of Nafion into CMA/N nanofibers at NH-modified ssDNA sample. The examined CMA/N hybrid nanofibers could be a promising DNA biosensor devices.