Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family

Abstract

Lamiaceae family is one of the members of important plants, using as a agents for alternative medicine treatments. Our aim was toinvestigate both antigenotoxic and genotoxic effects three different plants species belonging to Lamiaceae family: Stachys annua,Scutellaria salviifolia, Nepeta nuda in Amasya, using SMART (Drosophila melanogaster somatic mutation and recombination test)and also plasmid DNA interaction method, respectively. SMART analyses revealed that genotoxic effect of ethyl methanesulfonate(EMS) was eliminated by plant extract of S. salviifolia, indicating antigenotoxic effects. Different extract concentrations [(0.1, 0.25,0.50 and 1 % (v/v)] of other plants species decrased the flecks number on D. melanogaster but the results did not show any statisticalsignificance (p>0.05). In addition to SMART, interaction with pUC18 plasmid DNA was also analysed by using all concentrations(50 µg/mL-3.125 µg/mL) of plant extracts N. nuda and S. annua. The extracts did not cause changed in Form I DNA (supercoil) buttrasferred from Form II DNA (open circular) to Form III DNA. Moreover, there was no effect of S. salviifolia plant extracts on DNAand band density. SMART results were supported by DNA interaction analyses, cocncluding that S. salviifolia plants indicated thehigh-impact antigenotoxic effect, but not genotoxic effects.