Efficacy of amphotericin-b, clinicopathologic variables and oxidative stress markers in three staffordshire bull terrier dogs with visceral leishmaniasis

Visseral leishmaniasis li üç staffordshire bull terrier köpekte amfoterisin- b nin etkinliği, klinik-patolojik değişkenler ve oksidatif stres belirteçleri

Başak HANEDAN, Seckin ÖZKANLAR, Mehmet Kazim BORKU, Ahmet AYDIN, Yunusemre ÖZKANLAR, Ali Evren HAYDARDEDEOĞLU, Serkal GAZYAĞCI

Özet

BuçalışmadavisseralLeishmaniasisli(VL)üçStaffordshireBullTerrierköpekteLeishmaniaenfeksiyonuveAMB(amfoterisin-B)tedavisininhematolojik,biyokimyasalveoksidatifstresparametreleriüzerineetkilerinideğerlendirmekamaçlandı.Buköpeklerkilokaybı,halsizlikvederilezyonlarıilekliniğegetirildi.Kaninkala-azartestkitiVLliköpeklerdepozitifti.VisseralLeishmaniasisliolgularaAMB0.6-1.5mg/kg/haftadozunda4aysüreileuygulandı.Leishmaniaetkenleriolgu1ve2deALTdüzeyindeartışabağlıolarakkaraciğerhasarına,olgu3teeritrosit,hemoglobinvehematokritdüzeydeazalmayaveMDAdaartışaveGSH-PxdeazalmayanedenoldufakatVLliköpeklerdenormalürevekreatinindüzeyinebağlıolarakböbrekfonksiyonlarınıetkilemedi.AMBtedavisinekademeliyanıtgözlendi.TedaviseyrininsonundaVLliolgularklinikolarakiyileşti.Sonuçolarak6aysüreiletakiptekliniknüksolmamasınabağlıolarakvisseralLeishmaniasisitedavietmekiçin4aysüreliAMBuygulamasınınetkiliolabileceğikanısınavarıldı.

Abstract

This study was aimed at evaluating the effects of Leishmania infection and AMB (amphotericin-B) treatment onhematological, biochemical and oxidative stress parameters in three Staffordshire Bull Terrier dogs with visceralleishmaniasis (VL). These dogs were presented with weight loss, weakness, and cutaneous lesions. Canine kala-azardetection kit was positive in dogs with VL. AMB was administered to cases with VL at a dose of 0.6 to 1.5 mg/kg/week for 4months. Leishmania agents caused the liver injury due to increase in the ALT level in cases 1 and 2, decrease in erythrocyte,hemoglobin, and hematocrit level and increase inthe MDA, and decrease in the GSH-Px in case 3, but Leishmania agentsdid not affect the kidney functions due to normal urea and creatinine level in the dogs with VL. A gradual response to theAMB treatment was observed. At the end of treatment course, cases with VL were treatedclinically. It was concluded thattheAMB administrationfor 4 months might be effective to treat VL due to no clinical recurrence for 6-month follow-upperiod.