Impact of Kinalax 25 EC on Vital Organ Histomorphology, Blood Cell Structure and Brain Acetylcholinesterase Activity in Silver Barb (Barbonymus gonionotus)

Abstract

The objective of this research was to evaluate the effects of Kinalax 25 EC, anorganophosphate pesticide on silver barb (Barbonymus gonionotus). Histological bioassay of gill, liver and kidney of B. gonionotus, RBC count and acetylcholinesterase(AChE) activity were performed to evaluate the effects of the Kinalax 25 EC at cellularand enzymatic levels. The LC50 value of Kinalax 25 EC was estimated as 0.071 ppm forB. gonionotus. The remarkable changes included missing of gill lamellae, gill clubbing,hyperplasia, nuclear hypertrophy, vacuolation, glomerular expansion, increasing thediameter of renal tubules, hemorrhage, necrosis and pyknosis. Several changes inperipheral nuclear erythrocyte included large lymphocyte, dead cell, fusion of cell,binucleated cell, tear-shaped cell, ghost cell, senile cell and abnormal shape of cellwere found. The RBC count was significantly higher (P>0.01) in lower doses comparedto higher doses of pesticide in B. gonionotus. The level of AChE activities in B.gonionotus brain significantly decreased (P>0.01) in pesticides treated fish comparedto control. The result of this study reveals that the synthetic organophosphoruspesticide adversely affects the organ-specific histology, haematology and brainacetylcholinesterase activities of B. gonionotus.