Enzalutamide restores the testosterone effect on H19 expression in prostate cancer cells but not in exosomes

Abstract

Aim: Cellular or exosomal expression of an oncofetal lncRNA gene H19, was evaluated during androgen stimulation viadihydrotestosterone (DHT) or androgen receptor (AR) blockage via enzalutamide in cultured hormone-sensitive prostate cancer(PCa) cells which overexpress the androgen receptor (LNCaP-AR+) in the present study.Material and Methods: Cells were treated using DHT (10 nM) and/or enzalutamide (10 uM) for 24 hours. Cellular, and exosomalexpression of H19 was investigated using a quantitative polymerase chain reaction assay.Results: Our findings revealed that the mean cellular H19 expression decreased approximately 2.3 fold after androgen stimulationof PCa cells. Enzalutamide restored DHT effect with AR blockage, and we found increased H19 expression with the combined useof DHT, and enzalutamide compared with the levels in the control cells (p