N-acetylcysteine reduces oxidative stress on end-organs in an ischemia-reperfusion rat model

Abstract

Aim: In this study, we researched the effect of N-acetylcysteine (NAC) on parenchymal organs in ischemia-reperfusion injury in therat models.Material and Methods: Experimental animals divided into four groups: Sham group (Sh, n=6), Sham+NAC group (Sh+NAC, n=6),ischemia-reperfusion group (I/R, n=10) and N-acetylcysteine-given study group (I/R+NAC, n=10). For the Sham group and Sham +NAC group, only aortic exploration was performed. I/R group’s abdominal aortas were clamped just inferior to the renal artery for 4hours following. Then, the reperfusion period was allowed for one hour. I/R+NAC group received the same procedure as I/R groupand additionally treated with NAC intraperitoneally. After these procedures, all rats were sacrificed. The parenchymal organs wereexcised for biochemical, flow cytometric and histopathologic examination. The data were evaluated statistically.Results: In the I/R+NAC group, the MDA values in AC tissue were significantly lower than the I/R group (p:0.0032). MDA value inkidney tissue was significantly higher in the I/R group compared to the control groups (p:0.003). In the I/R+NAC group, the MDA valuewas significantly lower than the I / R group (p:0.0002). MPO values in lung tissue were found lower in the I/R+NAC group comparedto the I/R group, it was not statistically significant (p:0.4497). In the I/R+NAC group, it was found statistically significantly lowerthan both the control and I/R groups in Flow cytometric evaluation (p:0.0002). In histopathological evaluation, the leukocyte countobserved significantly higher in the I/R+NAC group compared to the control groups and was statistically significantly lower than theI/R group in histopathological evaluation (p:0,0004).Conclusion: NAC reduces ischemia and reperfusion injury in parenchymal organs and especially in the lungs in a rat model