The effect of perineural application of bupivacaine combined with sodium bicarbonate on the synatic nerve block in rabbits after sevofluran anesthesia

Abstract

Aim: The aim of this study was to investigate the effect of combining sodium bicarbonate with bupivacaine on prolonging peripheralnerve block time.Material and Method: Following the approval of the required Ethics Committee, 24 male New Zealand rabbit (4250-5350 g) wererandomized and divided into three groups. Group 1 sham n:8; Group 2 (bupivacaine): 0.5 mL of 0.5% bupivacaine (0.5 mg / kg) injectedinto the perineural area. n:8; and Group 3 (bupivacaine + sodium bicarbonate): 0.5 ml of 0.5% bupivacaine + sodium bicarbonate (125ml of 8.4% injected into the perineural area. n: 8.After the skin was closed in all groups, the paw pull response was monitored and recorded every 30 minutes until the sensory blockof the experimental animal returned back. Hot-plate test was used for analgesia evaluation. In addition, tissue histopathology wasexamined for histopathological evaluation of the injection site. Sensory block was evaluated with claw tightening test and claw pulltest (hot-plate) response. The measurements were carried out every 30 minutes for 120 minutes or until the block was completelyresolved.Results: 30., 60. and 90.min paw pull response in Group 2 and Group 3 showed statistically significant elongation when comparedto Group 1, this difference disappeared in 120 minutes. Compared to the sham group, the 30 min hot plate and claw pull responsewas significantly longer in group 3 (sodium bicarbonate and bupivacaine), this difference disappeared in 60 minutes (p = 0.018).Conclusion: When sodium bicarbonate and bupivacaine are combined, it was seen in this study that the sensory block was prolonged.We believe that the current results can be used as a guide for future studies