INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Özet

SUMMARYThis study aimed to evaluate the role of oxidative stress in toxicity inducedby BNL (benomyl) and its metabolite CBZ (carbendazim), whichare systemic broad-spectrum benzimidazole fungicides.The activities of superoxide dismutase (SOD), catalase (CAT), glutathioneperoxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyltranspeptidase (GGT) and glutathione-S-transferase (GST) as well as thelevels of malonyldialdehyde (MDA), being a lipid peroxidation markerand reduced glutathione (GSH) were measured in the liver, kidney, testisand brain tissues of the rats, after acute exposure of the two fungicides andtheir mixture.In all tissues, it was observed that MDA levels were increased andchanges in antioxidant defense system were occurred. The induction ofoxidative stress manifested as changes in the activity and/or levels of antioxidativeparameters has been suggested as the mechanism by which BNLand CBZ induces their toxic effects.ÖZETBu çalışmada, geniş spektrumlu sistemik benzimidazol grubu fungusidlerdenolan BNL (benomyl) ve metaboliti CBZ (carbendazim)’ nin toksiketki mekanizmalarında oksidatif stresin rolü araştırıldı. BNL, CBZ vekarışımlarına akut maruziyet sonrası sıçanların karaciğer, böbrek, testis vebeyin dokularında oksidatif stres göstergesi olarak lipid peroksidasyon sonürünü malondialdehid (MDA) ve indirgenmiş glutatyon (GSH) seviyeleri,süperoksid dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx),glutatyon redüktaz (GSH-Rd), gama glutamil transpeptidaz (GGT)ve glutatyon-S-transferaz (GST) antioksidan enzim aktivitelerinin tayiniyapıldı. Tüm dokularda MDA düzeylerinin arttığı ve antioksidan savunmasistemlerinde değişiklik olduğu gözlendi. BNL ve CBZ’nin toksisitesinde,antioksidan parametrelerin aktivite ve/veya düzeylerinde oksidatif stresinindüksiyonu sonucu görülen değişikliklerin rol oynadığı sonucuna varıldı.Key words: Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

Abstract

This study aimed to evaluate the role of oxidative stress in toxicity induced by BNL (benomyl) and its metabolite CBZ (carbendazim), which are systemic broad-spectrum benzimidazole fungicides.The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyl transpeptidase (GGT) and glutathione-S-transferase (GST) as well as the levels of malonyldialdehyde (MDA), being a lipid peroxidation marker and reduced glutathione (GSH) were measured in the liver, kidney, testis and brain tissues of the rats, after acute exposure of the two fungicides and their mixture.In all tissues, it was observed that MDA levels were increased and changes in antioxidant defense system were occurred. The induction of oxidative stress manifested as changes in the activity and/or levels of antioxidative parameters has been suggested as the mechanism by which BNL and CBZ induces their toxic effects. *Correspondence: [email protected]