The Effect of Rosmarinus Officinalis and Chemotherapeutic Etoposide on Glioblastoma (U87 MG) Cell Culture

Özet

AIM: To investigate whether high dose toxicities of etoposide can be overcome when used in combination with a natural compoundnamed Rosmarinus Officinalis for glioblastoma (GBM).MATERIAL and METHODS: The impact of Rosmarinus Officinalis in combination with etoposide on GBM U87 MG cells and MouseEmbryonic Fibroblast (MEF) cells was investigated. Both neutral red and 3-(4, 5-Dimethylthiazol-2-Yl)-2, 5-DiphenyltetrazoliumBromide (MTT) assays were employed to gauge cell viability.RESULTS: We observed that increased quantities of Rosmarinus Officinalis induced MEF cell proliferation while it inhibited thesurvival of GBM cells. Our results indicate that Rosmarinus Officinalis did not affect the cytotoxicity of etoposide on GBM cellcultures. In contrast, in the MEF cell cultures, Rosmarinus Officinalis induced proliferation and diminished the impact of etoposide.CONCLUSION: Rosmarinus Officinalis offers hope for developing new cancer treatment strategies. However, further studies areneeded to verify these results.