Evaluation of genotoxicity ınduced by medicinal plant jatropha gossypifolia in freshwater fish channa punctatus (bloch )

Özet

The genotoxic and mutagenic potential of medicinal plant Jatropha gossypifolia , was investigated in fresh water fish Channa punctatus in the present study using micronucleus assay and comet assay. Fishes were exposed at 20% and 40% of the 24 h -LC50 value of plant extract containing Apigenin (76.48 mg L-1 ) and crude latex (22.33 mg L-1 ). Both the test chemicals induced significant (P<0.05) genotoxic effects in exposed fishes at various exposure durations. The micronucleus induction was maximum at the highest concentration (40%) 96 h post exposure of Apigenin containing extract (0.107%) and Jatropha crude latex (0.302% ) in the peripheral blood of C. punctatus . A similar trend was observed for the DNA damage measured in terms of % tail DNA in the erythrocytes of C. punctatus in Apigenin containing extract (15.2 0% ) and Jatropha crude latex (21.17%). This study also explored the combined use of micronucleus assay and comet assay for in vivo laboratory studies using fresh water fishes for screening the genotoxic potential of medicinal plants.