The Effect of Stress Due to Nitrogen Limitation on Lipid Content of Phaeodactylum Tricornutum (Bohlin) Cultured Outdoor in Photobioreactor

Özet

Azot sınırlamasının, dışarı ortamda, tubular fotobiyoreaktörlerde kültüre alınan Bacillariophyceae sınıfına ait Phaeodactylum tricornutum (Bohlin)'da, hücre yoğunluğu, biyomas, klorofil a, total karoten, protein ve lipit miktarına etkisi araştırılmıştır. Phaeodactylum tricornutum, kontrol grubu olarak uygun ortamda ve aynı zamanda azotu %50 eksiltilmiş ortamda kültüre alınmıştır. Çalışmanın sonunda, %35.04 lipid, 0.980±0.02 gL-1 biyomas ve %8.87 protein içeriği saptanırken, kontrol grubunda %16.93 lipid, 1.036±0.025 gL-1 biyomas ve %31.05 protein belirlenmiştir. Sonuç olarak, azot sınırlamasının lipit miktarını artırırken, hücrenin biyomas, klorofil a, toplam karoten ve protein miktarında azalmaya neden olduğu gözlenmiştir

Abstract

The effect of nitrogen limitation on the cell density, biomass, chlorophyll a, total carotene, protein and lipid content of Phaeodactylum tricornutum (Bohlin), Bacillariophyceae, cultured in photobioreactors outdoor was investigated. Phaeodactylum tricornutum was cultivated in an appropriate medium as the control group, at the same time it was cultured in a medium of which nitrogen was reduced to 50%. At the end of the study, it was determined that 35.04% lipid with 0.980±0.02 gL-1 biomass and 8.87% protein content, meanwhile, in the control group, 16.93% lipid, 1.036±0.025 gLbiomass and 31.05% protein were determined. As a result, it was observed that while nitrogen limitation increased to the lipid amount, decreased the biomass and chlorophyll a, total carotene and protein of the cell