Dergiler / Etlik Veteriner Mikrobiyoloji Dergisi / 1999 / Cilt: 10 - Sayı: 2
DNA İmmunizasyonu
- Sayfa
- 73–85
- DOI
- —
Özet
DNA immunizasyonu birçok infeksiyöz ve infeksiyöz olmayan hastalıklardan korunma ve bunların sağaltımları için yeni ve umut vadeden bir yaklaşım sunmaktadır. Bu derlernede DNA aşısı üretimi, aşılama teknikleri ve DNA aşılarının kullanım alanları gözden geçirildi. DNA aşılarının avantaj ve dezavantajları da değerlendirilerek konvansiyonel aşılarla karşılaştırılması yapıldı.
Abstract
DNA immunization represents a new and promising approach for the prevention and therapy of many infectious and non-infectious diseases. With this approach, vaccines made of DNA are being developed as a form of gene therapy that use the patient's own cellular machinery to make foreign proteins that stimulate an immune response. Direct inoculations of vaccine DNAs (which are produced by doning DNA sequences encoding the protein or proteins to be used as immunogens into an eukaryotic expressian vector, in other words. by the help of the recombinant technology) into animals generate antibody, cytotoxic T cell (CTL) and protective immune respanses that have good longevity, with even single doses of DNA. Protective immune respanses can be generated by skin and muscle inoculations of DNA. Immunizations can be accomplished by injecting DNA in saline, or by using a gene gun topropel DNA-coated gold beads into cells. In this paper DNA vaccine production, vaccination techniques, current and potential uses of DNA immunization were reviewed. A comparison between DNA-mediated and conventional vaccination by evaluating its advantages and disadvantages were also mentioned.