Evaluation of the Effect of Daptomycin, a Glycopeptide Agent, on Intact Intervertebral Disc Tissue

Özet

AIM: To evaluate the effects of pre- and intra-operatively administered daptomycin (DAP) on the intact human primary intervertebraldisc tissue cells.MATERIAL and METHODS: Primary cell cultures were established using tissues obtained through decompressive laminectomy,traumatic intervertebral disc herniation excision, and posterior transpedicular stabilization. Non-drug-administered samples wereused as a control group. The samples treated with DAP formed the study group. Molecular assays for proliferation and geneexpression were performed. The obtained data were evaluated statistically, and results with a value of p<0.05 were accepted assignificant.RESULTS: While no reduction was observed in the proliferation, the gene expression of intact intervertebral disc tissue cells wastime-dependently decreased compared to the control group, and these results were reported to be statistically significant.CONCLUSION: This study observed the effect that a pharmaceutical preparation, which was used on intervertebral disc tissuebefore and after the operation, had on normal, healthy, and intact tissue. It concludes that alterations in the expression of genesinvolved in the anabolic and/or catabolic process, even in adjacent healthy tissue, may slow down the healing process of thedamaged tissue or cause undesired cell differentiation.