The Effects of Proanthocyanidin on Vasospasm After Experimental Subarachnoidal Hemorrhage in Rats

Özet

AIM: Delayed ischemic neurological deficit (DIND) and cerebral vasospasm (CV) remain the most common and debilitatingneurological complications following aneurysmal subarachnoidal hemorrhage (SAH). Many reports demonstrate the importance ofproanthocyanidins (PR) on the vascular system, including endothelium-dependent relaxation of blood vessels. These effects of PRon the cerebral vascular system were examined in this study.MATERIAL and METHODS: Fifty-two adult Sprague-Dawley male rats were used for the experimental double hemorrhage model.They were divided to control, sham, pre- and post-interventional treatment groups. 100 mg/kg PR was administered for thetreatment for respect to groups. Basilar artery diameter (BAD) and arterial wall thickness were measured and the apoptosis ratio ofthe endothelial cells was calculated. Arterial walls were examined electron microscopically (EM).RESULTS: There were significant differences between the groups except control and pre-SAH (p=0.37) and post-SAH and preSAH groups (p=0.15) with respect to BAD. According to arterial wall thickness, apoptosis ratio, and grading, there were significantdifferences between the groups except control and pre-SAH (p=0.85, p=0.49 and p=0.18 respectively) and SAH and post-SAH(p=0.08, p=0.21 and p=0.24 respectively) groups. EM findings revealed that pro-apoptotic and pro-necrotic degenerated endothelialcells with seldom vacuolization in post-SAH treatment group which were more serious in SAH group.CONCLUSION: Pre-SAH administration of PR induces better vasodilatation and protection of basilar artery (BA) from vasospasm(VS), which could yield neuroprotective and vasodilatator effects. In addition, PR appears to be involved in relieving oxidativedamage, with an antioxidant-antiapoptotic-antinecrotic effect that may contribute to vascular dilation.