Melatonin Attenuates White Matter Injury via Reducing Oligodendrocyte Apoptosis After Subarachnoid Hemorrhage in Mice

Özet

AIM: To determine whether melatonin (MLT) mitigates white matter (WM) injury by attenuating NOD-like receptor family pyrindomain-containing 3 (NLRP3)-associated oligodendrocyte apoptosis after subarachnoid hemorrhage (SAH).MATERIAL and METHODS: SAH model C57BL/6J mice were created using an endovascular perforation technique. The mice wereinjected intraperitoneally with MLT at doses of 50 mg/kg, 150 mg/kg and 300 mg/kg. The animals were evaluated for neurologicaloutcomes according to neurological score, brain water content, myelin degradation, amyloid precursor protein (APP) accumulation,apoptosis, and levels of NLRP3, caspase-1, interleukin-1β, Bcl-2, and Bcl-2-interacting mediator of cell death (Bim) expressionafter SAH.RESULTS: MLT at a dose of 50 mg/kg improved neurological score, alleviated brain edema, and reduced WM injury. In addition, lossof myelin basic protein (MBP) and accumulation of APP, and expression in the ipsilateral/left hemisphere were found after SAH, andwere reversed by MLT treatment. NLRP3 signal activation was found in microglia and apoptosis in oligodendrocytes were observedafter SAH. MLT reduced oligodendrocyte apoptosis by regulating Bim and Bcl-2.CONCLUSION: Results of this study indicated that MLT exerts a WM-protective effect in SAH pathophysiology, possibly byattenuating apoptosis in oligodendrocytes.