Effects of some michael type addition products on various cytokines

Özet

Bu gahsmamn amacı 2-[(2-nitro-l-fenilpropil)tiyo]benzoik asit (1), 2-[(2-nitro-l-feniletil)tiyometil] benzimidazol (2) ve 2-[(2-nitro-l-fenilpropil)tiyometil]benzimidazol (3) tiirevlerinin antisitokin aktivitelerinin insan primer hücrelerinde ve Mere hatlarmda araştinlmasıdır. Sitokinler inflamatuar basamaklarm oluşumunda Tümör Nekrozis Faktör-a (TNF-a), Interlökin (IL-lfi, IL-2, IL-4 ve IL-8), Gama interferon (IFN-y) He beraber çalisan habercilerdir. Hücre kültüründe yapılan bu çalismada bileşik 1, 2 ve 3 tiirevlerinin insan tarn kanı tarafından üretilen TNF-a, IL-J3, IL-8, IL-2, IL-4 ve IFN-y üzerindeki inhibisyon kapasiteleri ölgulmustür. Test sonuglan 1, 2 ve 3 tiirevlerinin lipopolisakkarit (LPS) He uyanlmis insan turn kanında IL-1J3, IL-8 ve TNF-a ve forbolasetat (PHA) He uyanlmis insan tiim kanında IL-2, IL-4 ve IFN-y salimmında doza bagimh inhibisyona sahip olduklarim göstermiştir.

Abstract

The aim of this research in to investigate the anticytokine activities of the 2-[(2-nitro-l-phenylpropyl)thio]benzoic acid (1), 2-[(2-nitro-l-phenylethyl)thiomethyl]benzimidazole (2) and 2-[(2-nitro-l-phenylpropyl)thiomethyl]benzimidazole (3) derivatives in human primary cells and cell lines. Cytokines are messengers for the regulation of the inflammatory cascades with Tumor Necrosis Factor-a (TNF-a), Interleukin (IL-1J3, IL-2, IL-4 and IL-8), Gamma Interferon (IFN-y) working synergistically. In this study which is performed in cell assay, inhibition capacity of compound 1, 2 and 3 derivatives againts TNF-a, IL-J3, IL-8, IL-2, IL-4 and IFN-y production by human whole blood have been measured . The test results were shown that 1, 2 and 3 derivatives have dose-dependent inhibitions on the release of IL-lfi, IL-8 and TNF-a in lipopolysaccharide (LPS) stimulated human whole blood and IL-2, IL-4 and IFN-y in phorbolacetate (PHA) stimulated in human whole blood.