Dergiler / Turkish Journal of Pharmaceutical Sciences / 2008 / Cilt: 5 - Sayı: 3
Effects of antituberculous drugs and their combinations on human polymorphonuclear leukocyte functions in vitro
- Sayfa
- 117–128
- DOI
- —
Özet
Günümüzde tuberküloz direncine karsi yeni antitüberküloz ilaçlar ve yeni kombinasyonlan geliştirilmektedir. Ancak bu ilaçların konagin immün sistemi üzerine olan etkileri henüz bilinmemektedir. Çalısmamızda primer antitüberküloz ilaçlar [izoniazid (İNH, 5jug/ml), rifampisin (RiF, 7jug/ml), pirazinamid (PZA 40jug/ml), etambutol (EMB, 7jug/ml), streptomicine (S, 25jug/ml)J, sekonder antituberkulous ilaçların [amikasin (A 24jug/ml), ofloksasin (OFLX, 2,9jug/ml), sikloserin (CYC, lOjug/ml), para-aminosalisilik asid (PAS, 90jug/ml), protionamid (PTH, l,6jug/ml), levofloxacin (LVFX, 2,8jug/ml)J ve klinik kombimasyonlarimn terapötik kombinasyonlarda, polimorf niiveli lökosit (PNL) fonksiyonlari ( fagositik ve Mere içi öldürme aktivitesi ) üzerine etkisi yaş ortalaması 25 olan 15 saglıklı gençte in vitro araştırılması amaçlanmistır. PNL’ler EDTA 'h venöz kandan Ficoll-Gradient yöntemi He ayrılmistır. Fagositoz ve Mere içi öldürme aktivitesi tayininde Alexander ve arkadaşlannın yöntemi modifiye edilerek kullanılmistır. Sekonder ilaçlardan A ve PAS kontrole göre fagositik aktiviteyi anlamlı artinrken OFLX Mere içi öldürme aktivitesini anlamlı artırmistır (p 0.05). Sonuç olarak, etkene olduğu kadar immiin sistem üzerine de olumlu etkileri bilinen antituberküloz ilaç ve kombinasyonlarının kullamlması PNL fonsiyonları iizerinde stimülatör etki göstererek tüberkülozlu hastaların tedavisine katkıda bulunabilir.
Abstract
Today new antibuberculous drugs and new combination regiments have been devevoped against the tuberculous resistance. However, the effects of these drugs on the immune system of the host has not been known yet. The aim of this study was to investigate the in vitro effects of primary antituberculous drugs [isoniazid (INH, 5jug/ml), rifampicine (RIF, 7jug/ml), pyrazinamide (PZA, 40jug/ml), ethambutol (EMB, 7jug/ml), streptomycine (S, 25jug/ml)J, secondary antituberculous drugs [amikacin (A, 24jug/ml), ofloxacin (OFLX, 2,9jug/ml), cyclocerine (CYC, lOjug/ml), para-aminosalicilic acid (PAS, 90jug/ml), prothionamide (PTH, l,6jug/ml), levofloxacin (LVFX, 2,8jug/ml)J and their combinations at therapeutic concentrations on polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) of 15 healthy young volunteers, whose average age was 25. PMNs were isolated by ficoll-hypaque gradient centrifugation method from venous blood with EDTA (O.lg/ml). Phagocytosis and intracellular killing activity were assayed by modifying Alexander’s method. A and PAS, which are secondary drugs that significantly increased the phagocytic activity, OFLX significantly increased the intracellular killing activity when compared with the control( drug-free) (p<0.05). The other primary and secondary drugs and their combinations did not significantly affect the phagocytic and intracellular killing activity when compared with the control( drug-free) (p>0.05). As a conclusion, the use of antituberculous drugs and their combinations whose possitive effects are known not only on the microorganism but also on the immune system maybe useful in the treatment of patients with tuberculosis by showing stimulatory effect on PMN functions .